Electric field mediated gene transfer
نویسندگان
چکیده
منابع مشابه
Electric field mediated gene transfer.
A simple physical chemical method of transferring genes into eucaryotic cells is described. Electric impulses in the intensity range of 5-10 kV/cm with a duration of 5-10 bs were found to appreciably increase the uptake of DNA into cells. After electric field treatment, stable transformants were obtained in a system containing mouse cells deficient in thymidine kinase and a plasmid DNA harbouri...
متن کاملElectric field-mediated gene transfer: characterization of DNA transfer and patterns of integration in lymphoid cells.
Southern analysis of individual transfectants generated by electroporation demonstrated a strong preference for the integration of DNA in low copy number even when electroporation was performed in the presence of increasing DNA concentrations. Although transfer of multiple DNA copies was detected at higher DNA concentrations (16 pmoles/ml or greater), the average gene copy number even at 36 pmo...
متن کاملControl by pulse parameters of electric field-mediated gene transfer in mammalian cells.
Electric field-mediated gene transfer in mammalian cells (electrotransformation) depends on the pulsing conditions (field intensity, pulse duration, number of pulses). The effect of these parameters was systematically investigated using the transient expression of the chloramphenicol acetyltransferase and the beta-galactosidase activities in Chinese hamster ovary cells. Pulsing conditions induc...
متن کاملSperm-mediated gene transfer.
Since 1989, a new method for the production of transgenic animals has been available, namely sperm-mediated gene transfer (SMGT), based on the intrinsic ability of sperm cells to bind and internalise exogenous DNA molecules and to transfer them into the oocyte at fertilisation. We first described the SMGT procedure in a small animal model, with high efficiency reported in the mouse. In addition...
متن کامل729-electric Gene Transfer into Culture Cells *
The electric field method of gene transfer via electroporation of cell membranes is gaining importance in cellular and molecular biology. In particular the electroporation technique provides the low copy number transfection required fqr genetic studies. As demonstrated with mouse lyoma cells, with lymphoid cell lines and with plant protoplast, the electric method has appreciable advantages comp...
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ژورنال
عنوان ژورنال: Biochemical and Biophysical Research Communications
سال: 1982
ISSN: 0006-291X
DOI: 10.1016/0006-291x(82)91531-5